【地域資源開発学科】生理活性リン脂質の合成法を確立

Sayumi Yasuda and Yukihiro Yamamoto

Efficient preparation methods for 1-lysophosphatidylcholine (1-LPC), a physiologically important compound, are lacking. Here, we established a method for 1-LPC preparation via Novozym® 435 (a lipase B from Candida antarctica)-catalyzed ethanolysis. Novozym® 435 showed sn-1 regiospecificity to phosphatidylcholine, although it does not exhibit regiospecificity to triacylglycerol. In particular, quantitative 1-LPC yields (96.5 ± 0.2 mol%) were reliably obtained in the presence of Novozym® 435 (100 wt% of PC), 97 % ethanol, in 72 h at 40 °C. During the reaction, acyl migration from 1-LPC to 2-LPC was rare. This novel synthetic method is expected to expand the practical applications of 1-LPC.

　当研究室卒業生である安田さん(2020年卒)の研究が論文化されました。
　ドコサヘキサエン酸やエイコサペンタエン酸などの高度不飽和脂肪酸は、1-LPCと呼ばれる脂質形態で運搬されます。本研究は、1-LPCを、酵素的に調製する方法を確立したものです。脂質化学や生化学分野における研究発展に応用できる成果です。