【地域資源開発学科】タケ培養細胞で有用 物質生産！

Naoki Kitaoka, Taiji Nomura,　Shinjiro Ogita,　Yasuo Kato

Rational metabolic-flow switching, which we proposed recently, is an effective strategy to produce an exogenous high-value natural product using transformed plant cells; the proof of this concept was demonstrated using bamboo (Phyllostachys nigra; Pn) cells as a model system. Pn cells were transformed to express 4-hydroxycinnamoyl-CoA hydratase/lyase of Pseudomonas putida KT2440 (PpHCHL), which catalyzes the formation of 4-hydroxybenzaldehyde and vanillin from p-coumaroyl-CoA and feruloyl-CoA, respectively. The PpHCHL-transformed cells accumulated glucose conjugates of 4-hydroxybenzoic acid and vanillic acid, indicating that the PpHCHL products (aldehydes) were further metabolized by inherent enzymes in the Pn cells. The production titers of 4-hydroxybenzoic acid glucose ester, vanillic acid glucose ester, and 4-hydroxybenzoic acid glucoside reached 1.7, 0.17, and 0.14 g/L at the maximum, respectively. These results proved the versatility of Pn cells for producing vanillin-related compounds based on rational metabolic-flow switching.

新たな代謝ルートを植物細胞の中で構築できる合理的代謝フロースィッチング注）の手法を、フェニルプロパノイド系化合物生産の宿主として独自に開発したタケ培養細胞に応用しました。Pseudomonas putidaの4-hydroxycinnamoyl-CoA hydratase/lyaseを発現する組換タケ培養細胞は、有用な天然香料であるバニリン関連代謝物を生産できることを示しました。本研究は、富山県立大学工学部生物工学科植物機能工学講座との共同研究成果です。